BIOTECHNOLOGY LEARNING CURVES SERIES: Introduction to Vaccine Production (Part 7)

Bacterial Vaccines: Preparation of Inoculum Media (Tryptic Soil Broth)

The inoculums media used for the fermentation process is called Triptych Soil Broth (TSB). 15 L TSB media is used to inoculate into 200 L fermentation media. To produce 1 L of TSB solution, 30g of TSB powder is diluted with 1L water. The figures above show the process of making the TSB inoculums media.

After that, the colonies of bacteria will be transfered to the 15L inoculum TSB media and incubated for 24 hours. This step is to enable the bacteria to further propagate before being transfered into the bioreactor.