BIOTECHNOLOGY LEARNING CURVES SERIES: Introduction to Vaccines Production (Part 6)

Bacterial Vaccines: Preparation of Seed Bacteria

Eight Pasteurella multocida strains which have different antigenic structures seed are taken out from the freezer and allow standing for half an hour to thaw the seed. Then, it was sub cultured on the blood agar and incubated at 37^oC for 24 hours. Due to the rich nutrient provided from the blood agar, there should be a good growth of the bacteria. The purity can be observed directly according to the characteristic, morphology and smell. If it found out contains the contaminant, another sub culture is done to further purify the bacteria. After that, 20 pure single colonies from the plate are inoculated into the universal bottles containing TSB and incubated at 37^oC for 4 hours.